Journal: Optics Express

Article Title: A versatile diffractive maskless lithography for single-shot and serial microfabrication

doi: 10.1364/OE.18.011754

Figure Lengend Snippet: BSA-FITC solution exposed to three time-averaged patterns simultaneously at 110 mW for 10 s. (a) Real-time microscope image captured during fabrication. (b) Fluorescent image of patterned protein after sample wash with 1x PBS. (c) SEM image of sample after a chemical desiccation process. Predicted feature dimensions were assessed using area measurements from both the fluorescent and SEM images. The fluorescent measurements indicated the features were ~6% larger than desired, while the SEM measurements indicated they were ~6% smaller than desired; demonstrating an accurate tolerance for pattern transfer. Next, a BSA-FITC solution spiked with avidin conjugated FITC was patterned. Biotin conjugated with ATTO 655 was applied to the resulting BSA/avidin structures for 20 min to assess the retention of avidin binding ability. Fluorescent microscope images were taken using (d) FITC and (e) Cy5 filter sets. A combined fluorescent image (f) reveals the functionality of the substrate after patterning. All scale bars are 50 µm.

Article Snippet: Next, a 33 mg mL −1 solution was made by dissolving 2 mg of FITC conjugated avidin (Rockland A003-02) in 60 µL of 1x PBS.

Techniques: Microscopy, Avidin-Biotin Assay, Binding Assay